Anti-NFIB antibody
| 英文名称 | NFIB |
| 中文名称 | 核因子1B抗体 |
| 别 名 | CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; NFIB; NFIB_HUMAN; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; NFIB2; NFIB3; HMGIC/NFIB. |
DATASHEET
Host:Rabbit
Target Protein:NFIB
IR:Immunogen Range:1-100/420
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4781
Swiss Prot:O00712
Source:KLH conjugated synthetic peptide derived from human NFIB/NF1B2:1-100/420
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Ovary (Mouse) Lysate at 40 ug
Lane 5: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:293T Cell (Human) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
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