Anti-ST18 antibody (bs-12188R)

Anti-ST18 antibody

DATASHEET

Host:Rabbit

Target Protein:ST18

IR:Immunogen Range:451-550/1047

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9705

Swiss Prot:O60284

Source:KLH conjugated synthetic peptide derived from human ST18/Znf387:451-550/1047 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ST18 is a 1,047 amino acid nuclear protein that is expressed at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Containing six C2HC-type zinc fingers and an SMC domain, ST18 is a repressor protein that binds to DNA sequences containing a specific bipartite element. ST18 is considered a transcription factor that represses basal transcription activity from target promoters and Inhibits colony formation in cultured breast cancer cells. ST18 is encoded by a gene located on human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3ug/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- ST18 (bs-12188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 110 kD

Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- ST18 (bs-12188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated(bs-12188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining