Anti-phospho-CSNK1G 1+2+3 ( Tyr263) antibody
| 英文名称 | phospho-CSNK1G 1+2+3 ( Tyr263) |
| 中文名称 | 磷酸化酪蛋白激酶1γ1+γ2+γ3抗体 |
| 别 名 | Casein kinase I isoforms gamma 1+2+3 (phospho Y263); p-CK1g2(phospho Y263); p-CSNK1G1(phospho Y263); p-CSNK1G2(phospho Y263); p-CSNK1G3(phospho Y263); Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3; KC1G1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-CSNK1G 1+2+3 ( Tyr263)
IR:Immunogen Range:ER(p-Y)QK
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:53944
Swiss Prot:Q9HCP0
Source:KLH conjugated synthesised phosphopeptide derived from human Casein kinase I isoforms gamma 1+2+3 around the phosphorylation site of Tyr263:ER(p-Y)QK
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. They can phosphorylate a large number of proteins. Participates in Wnt signaling.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-phospho-CSNK1G 1+2+3 ( Tyr263) (bs-12426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CSNK1G 1+2+3 ( Tyr263)) Polyclonal Antibody, Unconjugated (bs-12426R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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