Anti-phospho-beta Actin (Tyr53) antibody (bs-12581R)

Anti-phospho-beta Actin (Tyr53) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-beta Actin (Tyr53)

IR:Immunogen Range:DS(p-Y)VG

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:60

Swiss Prot:P60709

Source:KLH conjugated synthesised phosphopeptide derived from human beta Actin around the phosphorylation site of Tyr53:DS(p-Y)VG 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
ICC(1:100)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Primary:
Anti- phospho-beta Actin (Tyr53) (bs-12581R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- phospho-beta Actin (Tyr53) (bs-12581R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta Actin (Tyr53)) Polyclonal Antibody, Unconjugated (bs-12581R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.