Anti-HADHSC antibody
| 英文名称 | HADHSC |
| 中文名称 | 短链L-3羟烷基辅酶A脱氢酶抗体 |
| 别 名 | HAD; HADH; HADH1; HADHSC; HCDH; HCDH_HUMAN; HHF4; HydroJNCacyl CoA dehydrogenase; HydroJNCacyl-coenzyme A dehydrogenase; hydroJNCacyl-coenzyme A dehydrogenase, mitochondrial; L 3 hydroJNCacyl Coenzyme A dehydrogenase short chain; M SCHAD; Medium and short chain L 3 hydroJNCacyl coenzyme A dehydrogenase; Medium and short-chain L-3-hydroJNCacyl-coenzyme A dehydrogenase; MGC8392; mitochondrial; MSCHAD; OTTHUMP00000162626; OTTHUMP00000219688; SCHAD; Short chain 3 hydroJNCacyl CoA dehydrogenase mitochondrial; short chain 3-hydroJNCacyl-coa dehydrogenase; Short-chain 3-hydroJNCacyl-CoA dehydrogenase. |
DATASHEET
Host:Rabbit
Target Protein:HADHSC
IR:Immunogen Range:241-314/314
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3033
Swiss Prot:Q16836
Source:KLH conjugated synthetic peptide derived from human HADHSC:241-314/314
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene is a member of the 3-hydroJNCacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain3-hydroJNCacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq.]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- HADHSC (bs-10020R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-HADHSC (bs-10020R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HADHSC Polyclonal Antibody, Unconjugated(bs-10020R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HADHSC) Polyclonal Antibody, Unconjugated (bs-10020R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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