Anti-MCT1 antibody
| 英文名称 | MCT1 |
| 中文名称 | 单羧酸转运蛋白-1抗体 |
| 别 名 | monocarboJNClate transporter 1; Malignant T cell amplified sequence 1; MCT 1; MCT1; MCTS 1; MCTS1; Oncogene MCT 1; Oncogene MCT1; SLC16A1; FLJ36745; HHF7; MCT; MGC44475; monocarboJNClate transporter; MonocarboJNClate transporter isoform 1; MonocarboJNClic acid transporter 1; SLC16A1; SLC16A1 protein; Solute carrier family 16 (monocarboJNClic acid transporters) member 1; Solute carrier family 16 member 1 (monocarboJNClic acid transporter 1); Solute carrier family 16 member 1; MOT1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MCT1
IR:Immunogen Range:251-350/500
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6566
Swiss Prot:P53985
Source:KLH conjugated synthetic peptide derived from human MCT1:251-350/500
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a proton-linked monocarboJNClate transporter that catalyzes the movement of many monocarboJNClates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:HepG2 (Human)cell Lysate at 40 ug
Primary: Anti-MCT1(bs-10249R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Stomach (Mouse) Lysate at 40 ug
Lane 3: Heart (Mouse) Lysate at 40 ug
Lane 4: Liver (Rat) Lysate at 40 ug
Lane 5: Stomach (Rat) Lysate at 40 ug
Lane 6: Heart (Rat) Lysate at 40 ug
Primary: Anti-MCT1 (bs-10249R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCT1) Polyclonal Antibody, Unconjugated (bs-10249R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-MCT1 Antibody, conjugated (bs-10249R-AF488) 1:400, 1.5 hours at 37°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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