Anti-FHOD1 antibody (bs-13158R)

Anti-FHOD1 antibody

DATASHEET

Host:Rabbit

Target Protein:FHOD1

IR:Immunogen Range:601-700/1164

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:29109

Swiss Prot:Q9Y613

Source:KLH conjugated synthetic peptide derived from human FHOD1:601-700/1164 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of protein products known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing actin filament assembly. The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. FHOD1 induces the formation of and associates with bundled actin stress fibers in response to the activity of the Rho-ROCK cascade. It influences several cellular activities including cell migration, cytoskeletal arrangement, signal transduction and gene expression.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Muscle (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- FHOD1 (bs-13158R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- FHOD1 (bs-13158R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(bs-13158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining