Anti-phospho-FHOD1 (Thr1141) antibody
| 英文名称 | phospho-FHOD1 (Thr1141) |
| 中文名称 | 磷酸化肢体畸形相关蛋白FHOD1抗体 |
| 别 名 | FHOD1 (phospho T1141); p-FHOD1 (phospho T1141); FH1/FH2 domain containing protein; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 domain containing protein 1; Formin homology 2 domain-containing protein 1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-FHOD1 (Thr1141)
IR:Immunogen Range:RR(p-T)LK
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:29109
Swiss Prot:Q9Y613
Source:KLH conjugated synthesised phosphopeptide derived from human FHOD1 around the phosphorylation site of Thr1141:RR(p-T)LK
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of protein products known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing actin filament assembly. The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. FHOD1 induces the formation of and associates with bundled actin stress fibers in response to the activity of the Rho-ROCK cascade. It influences several cellular activities including cell migration, cytoskeletal arrangement, signal transduction and gene expression.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-FHOD1 (Thr1141) (bs-13159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-FHOD1 (Thr1141) (bs-13159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated(bs-13159R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(bs-13159R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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