Anti-HERC6 antibody (bs-15463R)

Anti-HERC6 antibody     

DATASHEET

Host:Rabbit

Target Protein:HERC6

IR:Immunogen Range:751-850/1022

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:55008

Swiss Prot:Q8IVU3

Source:KLH conjugated synthetic peptide derived from human HERC6:751-850/1022 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-HERC6(bs-15463R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kD
Observed band size: 115kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (bs-15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.