Anti-CYP2U1 antibody (bs-14153R)

Anti-CYP2U1 antibody     

DATASHEET

Host:Rabbit

Target Protein:CYP2U1

IR:Immunogen Range:151-250/544

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:113612

Swiss Prot:Q7Z449

Source:KLH conjugated synthetic peptide derived from human CYP2U1:151-250/544 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooJNCgenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a hydroJNClase that metabolizes arachidonic acid, docosahexaenoic acid, and other long chain fatty acids. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Thymus (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-CYP2U1(bs-14153R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 63kD

Sample:
Hela Cell(Human)Lysate at 30 ug
Lovo Cell(Human)Lysate at 30 ug
Primary: Anti- CYP2U1 (bs-14153R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62kD
Observed band size: 62kD

Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2U1) Polyclonal Antibody, Unconjugated (bs-14153R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2U1) Polyclonal Antibody, Unconjugated (bs-14153R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.