Anti-phospho-EIF4B (Ser406) antibody (bs-14551R)

Anti-phospho-EIF4B (Ser406) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-EIF4B (Ser406)

IR:Immunogen Range:HP(p-S)WR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1975

Swiss Prot:P23588

Source:KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD

Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD

Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.