Anti-TRIT1 antibody (bs-17134R)

Anti-TRIT1 antibody

DATASHEET

Host:Rabbit

Target Protein:TRIT1

IR:Immunogen Range:121-220/467

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:54802

Swiss Prot:Q9H3H1

Source:KLH conjugated synthetic peptide derived from human TRIT1:121-220/467 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Uterus(Mouse) Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Sample: Lung(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Sample: Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(bs-17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIT1) Polyclonal Antibody, Unconjugated (bs-17134R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.