Anti-MPND antibody (bs-17737R)

Anti-MPND antibody

DATASHEET

Host:Rabbit

Target Protein:MPND

IR:Immunogen Range:101-200/471

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:68047

Swiss Prot:Q3TV65

Source:KLH conjugated synthetic peptide derived from human MPND:101-200/471 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Proteases are enzymes that are involved in protein catabolism by hydrolyzing the peptide bonds between amino acids in a polypeptide chain. MPND (MPN domain-containing protein) is a 471 amino acid protein that is thought to be a protease. Expressed as two isoforms produced by alternative splicing, MPND contains a JAMM motif and one MPN domain. The gene that encodes MPND maps to human chromosome 19, which consists of around 63 million bases with over 1,400 genes, making up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fcα receptors. Key genes for eye color and hair color also map to chromosome 19.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
SH-SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- MPND (bs-17737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (bs-17737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (bs-17737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (bs-17737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.