Anti-Nesprin 3 antibody
| 英文名称 | Nesprin 3 |
| 中文名称 | 突触核膜蛋白3抗体 |
| 别 名 | C14orf49; Chromosome 14 open reading frame 49; FLJ25605; MGC75267; Nesp3; Nesprin3; Nesprin-3; NET53; Nuclear Envelope Spectrin Repeat Protein 3; Spectrin Repeat Containing, Nuclear Envelope Family Member 3; SYNE3; SYNE3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Nesprin 3
IR:Immunogen Range:201-300/975
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:161176
Swiss Prot:Q6ZMZ3
Source:KLH conjugated synthetic peptide derived from human Nesprin 3:201-300/975
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Hepg2(Human) Cell Lysate at 30 ug
Primary: Anti-Nesprin 3 (bs-19209R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 112 kD
Observed band size: 112 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (bs-19209R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (bs-19209R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nesprin 3) Polyclonal Antibody, Unconjugated (bs-19209R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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