Anti-Epac2 antibody (bs-10955R)

Anti-Epac2 antibody

DATASHEET

Host:Rabbit

Target Protein:Epac2

IR:Immunogen Range:151-250/1011

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:11069

Swiss Prot:Q8WZA2

Source:KLH conjugated synthetic peptide derived from human Epac2:151-250/1011 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the ras family GTPases Rap1 and Rap2 by promoting GTP binding in a cAMP-dependent manner (1,2). Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission (3). Human EPAC2 contains at least 31 exons and maps to chromosome 2q31 (4). The 4.4-kb Epac2 transcript is prominent in brain and adrenal gland (5,6). Within the brain, expression is strong in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus and cerebellum (5,6).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HL60 (Human) Cell Lysate at 30 ug
Primary: Anti- Epac2 (bs-10955R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-aEpac2 (bs-10955R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD

Protein:
liver(mouse) lysate at 40ug;
brain(mouse) lysate at 40ug;
Primary: rabbit Anti-Epac2 (bs-10955R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 116 kD
Observed band size: 116 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Epac2 Polyclonal Antibody, Unconjugated(bs-10955R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining