Anti-NRBP2 antibody
英文名称 | NRBP2 |
中文名称 | 核受体结合蛋白2抗体 |
别 名 | BC011468; DKFZp434P086; MGC12117; MGC138699; MGC18994; NRBP2; NRBP2_HUMAN; Nuclear receptor binding protein 2; Nuclear receptor-binding protein 2; PP9320; Transformation related gene 16 protein; Transformation-related gene 16 protein; transformation-related protein 16; TRG 16; TRG-16; TRG16. |
DATASHEET
Host:Rabbit
Target Protein:NRBP2
IR:Immunogen Range:401-501/501
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:340371
Swiss Prot:Q9NSY0
Source:KLH conjugated synthetic peptide derived from human NRBP2:401-501/501
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Cerebellum (mouse) Lysate at 40 ug
Primary: Anti-NRBP2(bs-19344R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample: brain (mouse) Lysate at 40 ug
Primary: Anti-NRBP2(bs-19344R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(bs-19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(bs-19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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