Anti-Angiotensin III antibody
| 英文名称 | Angiotensin III |
| 中文名称 | 血管紧张素III抗体 |
| 别 名 | ANGT_HUMAN; Angiotensin-1; Angiotensin I; Ang I; Angiotensin-2; Angiotensin II; Ang II; Ang III; Angiotensin-3; Angiotensin III; Angiotensin3; Angiotensin 3; Angiotensin-4; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 2-8; Angiotensin 3-8; Angiotensin 1-9; Angiotensin 1-10; Angiotensinogen; Serpin A8; Angiotensin IV; Ang IV; ANHU; Des-Asp[1]-angiotensin II; SERPINA8. |
DATASHEET
Host:Rabbit
Target Protein:Angiotensin III
IR:Immunogen Range:
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:183
Swiss Prot:P01019
Source:KLH conjugated synthetic peptide of human Angiotensin III (RVYIHPF):
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Angiotensin III is an eptapeptide drived from cleavage of full length angiotensin (AGT). AGT is a polypeptide vasopressor hormone formed by the catalytic action of renin, which is released mainly by the kidneys in response to low blood volume or low sodium content in the body. The juxtaglomerular cells release renin when juxtaglomerular apparatus decreases in the kidneys. Renin (called angiotensinogenase) is an enzyme of the hydrolase class that catalyzes cleavage of the leucine leucine bond in angiotensinogen (a serum globulin) to generate angiotensin I in the liver. Angiotensin I acts mainly as a precursor to angiotensin II, which is a vasopressor and stimulator of aldosterone secretion by the adrenal cortex, and also functions as a neurotransmitter. Angiotensin III (heptapeptide) is a degradation product of angiotensin II, and has less vasopressor activity than the parent compound. However, Angiotensin III does stimulate aldosterone release.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Plasma (Mouse) at 20 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Lane 4: Adrenal gland (Mouse) Lysate at 40 ug
Lane 5: Adrenal gland (Rat) Lysate at 40 ug
Lane 6: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Angiotensin III (bs-20101R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52/60 kD
Observed band size: 52/60 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin III) Polyclonal Antibody, Unconjugated (bs-20101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin III) Polyclonal Antibody, Unconjugated (bs-20101R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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