Anti-PPP2R5E antibody
| 英文名称 | PPP2R5E |
| 中文名称 | 蛋白磷酸酶2调节亚基5E抗体 |
| 别 名 | 2A5E_HUMAN; Epsilon isoform of regulatory subunit B56 protein phosphatase 2A; PP2A B subunit B' epsilon; PP2A B subunit B' epsilon isoform; PP2A B subunit B56 epsilon; PP2A B subunit B56 epsilon isoform; PP2A B subunit isoform B''-epsilon; PP2A B subunit isoform B'-epsilon; PP2A B subunit isoform B56-epsilon; PP2A B subunit isoform PR61-epsilon; PP2A B subunit isoform R5-epsilon; PP2A B subunit PR61 epsilon; PP2A B subunit PR61 epsilon isoform; PP2A B subunit R5 epsilon; PP2A B subunit R5 epsilon isoform; PPP2R5E; Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform; Protein phosphatase 2 regulatory subunit B' epsilon isoform; Regulatory subunit B of protein phosphatase 2 epsilon isoform; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon. |
DATASHEET
Host:Rabbit
Target Protein:PPP2R5E
IR:Immunogen Range:121-220/467
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5529
Swiss Prot:Q16537
Source:KLH conjugated synthetic peptide derived from human PPP2R5E:121-220/467
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- PPP2R5E (bs-19971R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Epithelium (Mouse) Lysate at 40 ug
Primary: Anti-PPP2R5E(bs-19971R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP2R5E) Polyclonal Antibody, Unconjugated (bs-19971R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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