Anti-PPM1E antibody (bs-21063R)

Anti-PPM1E antibody

DATASHEET

Host:Rabbit

Target Protein:PPM1E

IR:Immunogen Range:321-420/764

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:22843

Swiss Prot:Q8WY54

Source:KLH conjugated synthetic peptide derived from human PPM1E:321-420/764 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Zebrafish
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-PPM1E (bs-21063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Sample:
Olfactory buib(Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- PPM1E (bs-21063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-PPM1E (bs-21063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPM1E) Polyclonal Antibody, Unconjugated (bs-21063R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.