Anti-Synaptophysin antibody (bs-23504R)

Anti-Synaptophysin  antibody

DATASHEET

Host:Rabbit

Target Protein:Synaptophysin

IR:Immunogen Range:201-300/313

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6855

Swiss Prot:P08247

Source:KLH conjugated synthetic peptide derived from human Synaptophysin :201-300/313 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti- Synaptophysin (bs-23504R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 39 kD

Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Synaptophysin (bs-23504R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 39 kD

Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti-Synaptophysin (bs-23504R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 39 kD

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Synaptophysin (bs-23504R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 39 kD