Anti-IL1RAPL1 antibody
| 英文名称 | IL1RAPL1 |
| 中文名称 | 白介素1受体相关蛋白样1前体蛋白抗体 |
| 别 名 | IL 1 RAPL 1; IL 1RAPL 1; IL-1-RAPL-1; IL-1RAPL-1; IL1R8; IL1RAPL 1; IL1RAPL; IL1RAPL-1; IL1RAPL1; Interleukin 1 receptor 8; Interleukin 1 receptor accessory protein like 1; Interleukin-1 receptor accessory protein-like 1; IRPL1_HUMAN; Mental retardation X linked 10; Mental retardation X linked 21; Mental retardation X linked 34; MRX 21; MRX 34; MRX10; MRX21; MRX34; Oligophrenin 4; Oligophrenin-4; Oligophrenin4; OPHN 4; OPHN4; Three immunoglobulin domain containing IL 1 receptor related 2; Three immunoglobulin domain-containing IL-1 receptor-related 2; TIGIRR 2; TIGIRR-2; TIGIRR2 antibody X linked interleukin 1 receptor accessory protein like 1; X-linked interleukin-1 receptor accessory protein-like 1. |
DATASHEET
Host:Rabbit
Target Protein:IL1RAPL1
IR:Immunogen Range:301-400/696
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:11141
Swiss Prot:Q9NZN1
Source:KLH conjugated synthetic peptide derived from human IL1RAPL1:301-400/696
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the interleukin 1 receptor family and is similar to the interleukin 1 accessory proteins. It is most closely related to interleukin 1 receptor accessory protein-like 2 (IL1RAPL2). This gene and IL1RAPL2 are located at a region on chromosome X that is associated with X-linked non-syndromic mental retardation. Deletions and mutations in this gene were found in patients with mental retardation. This gene is expressed at a high level in post-natal brain structures involved in the hippocampal memory system, which suggests a specialized role in the physiological processes underlying memory and learning abilities.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Hippocampal (Mouse) Lysate at 40 ug
Primary: Anti-IL1RAPL1 (bs-0445R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 108 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL1RAPL1 Polyclonal Antibody, Unconjugated(bs-0445R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:TM4(blue).
Primary Antibody: Rabbit Anti-IL1RAPL1 antibody(bs-0445R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (bs-0445R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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