Anti-MMP-3 antibody
| 英文名称 | MMP-3 |
| 中文名称 | 基质金属蛋白酶3抗体 |
| 别 名 | matrix metalloproteinase-3; Transin-1; SL-1; SL 1; SL1; Stromelysin-1 precursor; MMP3 Stromelysin-1; EMS-2; Matrix metalloproteinase 3; Matrix metalloproteinase-3; Matrix metalloproteinase 3 preproprotein; MGC126102; MGC126103; MGC126104; MMP 3; MMP3; Progelatinase; Proteoglycanase;STMY; STMY1; STR1; Stromelisin 1; Stromelysin 1 progelatinase; Transin 1; MMP3_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MMP-3
IR:Immunogen Range:401-477/477
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4314
Swiss Prot:P08254
Source:KLH conjugated synthetic peptide derived from human MMP3:401-477/477
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Sheep
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
HepG2(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-MMP-3 (bs-0413R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42/52 kD
Observed band size: 52 kD
Sample:Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- MMP-3 (bs-0413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42/52kD
Observed band size: 52kD
Tissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-3 Polyclonal Antibody, Unconjugated(bs-0413R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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