Anti-Neuropeptide Y antibody (bs-0071R)

Anti-Neuropeptide Y antibody

DATASHEET

Host:Rabbit

Target Protein:Neuropeptide Y

IR:Immunogen Range:29-64/97

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4852

Swiss Prot:P01303

Source:KLH conjugated synthetic peptide derived from human NPY:29-64/97 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-0071R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropeptide Y) Polyclonal Antibody, Unconjugated (bs-0071R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Formalin-fixed and paraffin-embedded rat brain tissue labeled with Rabbit Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-0071R) at 1:400 followed byconjugation to the secondary antibody and DAB staining