Anti-Podoplanin antibody
| 英文名称 | Podoplanin |
| 中文名称 | 平足蛋白/淋巴管内皮细胞蛋白抗体 |
| 别 名 | Podoplanin /gp36; Podoplanin Protein; AGGRUS; GLYCOPROTEIN 36 KD; Podoplanin; Glycoprotein 36; gp 36; GP 38; GP 40; gp36; GP38; GP40; HT1A 1; HT1A1; hT1alpha1; hT1alpha2; Lung type I cell membrane associated glycoprotein; Lung type I cell membrane associated glycoprotein isoform a; Lung type I cell membrane associated glycoprotein T1A 2; OTS 8; OTS8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26; PA2.26 antigen; PDPN; Podoplanin; T1 alpha; T1 ALPHA GENE; T1A; TIA 2; TIA2. |
DATASHEET
Host:Rabbit
Target Protein:Podoplanin
IR:Immunogen Range:91-166/166
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:14726
Swiss Prot:Q62011
Source:KLH conjugated synthetic peptide derived from mouse Podoplanin:91-166/166
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Cross Reactive Species:Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- Podoplanin (bs-1048R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (bs-1048R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Podoplanin Protein/gp36 Polyclonal Antibody, Unconjugated(bs-1048R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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