Anti-MAP1LC3A antibody
| 英文名称 | MAP1LC3A |
| 中文名称 | 自噬微管相关蛋白轻链3抗体 |
| 别 名 | ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A and 1B, light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:MAP1LC3A
IR:Immunogen Range:75-121/121
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:84557
Swiss Prot:Q9H492
Source:KLH conjugated synthetic peptide derived from human MAP1LC3A:75-121/121
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2μg/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:Testis (Mouse) Lysate at 30 ug
Primary: Anti-MAP1LC3A (bs-1534R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14 kD
Observed band size: 18 kD
Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LC3 α/MAP1A/MAP LC3 Alpha/Beta Polyclonal Antibody, Unconjugated (bs-1534R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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