Anti-Muscarinic Acetylcholine receptor M3 antibody
| 英文名称 | Muscarinic Acetylcholine receptor M3 |
| 中文名称 | 毒蕈碱型乙酰胆碱受体M3抗体 |
| 别 名 | Muscarinic Acetylcholine receptor M3; cholinergic receptor, muscarinic 3; CHRM 3; CHRM3; HM 3; HM3; HM3; EGBRS; M3 muscarinic receptor; muscarinic 3; m3 muscarinic acetylcholine receptor; ACM3_HUMAN; muscarinic receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor; Acetylcholine receptor(M3). |
DATASHEET
Host:Rabbit
Target Protein:Muscarinic Acetylcholine receptor M3
IR:Immunogen Range:481-589/589
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1131
Swiss Prot:Q9ERZ3
Source:KLH conjugated synthetic peptide derived from human ChRM3:481-589/589
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Anti-Muscarinic Acetylcholine receptor (bs-1289R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor(M3) Polyclonal Antibody, Unconjugated (bs-1289R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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