Anti-E2F4 antibody (bs-1399R)

Anti-E2F4 antibody 

DATASHEET

Host:Rabbit

Target Protein:E2F4

IR:Immunogen Range:1-150/413

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1874

Swiss Prot:Q16254

Source:KLH conjugated synthetic peptide derived from human E2F4:1-150/413 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IP(1:20-100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-E2F4 (bs-1399R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 44 kD
Observed band size : 44 kD

CK18 was immunoprecipitated from human hela cells lysate with bs-1399R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 μg (Input).
Lane 2: bs-1399R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F4) Polyclonal Antibody, Unconjugated (bs-1399R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.