Anti-BRG-1 antibody
| 英文名称 | BRG-1 |
| 中文名称 | 抑癌基因SNF2β抗体 |
| 别 名 | BRG1; ATP dependent helicase SMARCA4; BAF 190; BAF190; BAF190A; Brahma protein homolog 1; Brahma protein like 1; BRG 1; BRG 1 protein; BRG1; BRG1 associated factor 190A; BRG1 protein; BRM/SWI2 related gene 1; Global transcription activator homologous sequence; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; Nuclear protein GRB1; Probable global transcription activator SNF2L4; Protein brahma homolog 1; Protein BRG1; SMARC A4; SMARCA 4; SMARCA4; SNF 2; SNF 2B; SNF2;;brahma-related gene 1; SNF2 beta; SNF2 like 4; SNF2B; SNF2beta; SNF2L4; SNF2LB; SWI 2; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4; SWI2; Transcription activator BRG1. |
DATASHEET
Host:Rabbit
Target Protein:BRG-1
IR:Immunogen Range:51-167/1647
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6597
Swiss Prot:P51532
Source:KLH conjugated synthetic peptide derived from human SMARCA4:51-167/1647
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3ug/test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRG-1) Polyclonal Antibody, Unconjugated (bs-1408R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BRG-1) Polyclonal Antibody, Unconjugated (bs-1408R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-BRG-1 antibody (bs-1408R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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