Anti-IL-23R antibody (bs-1460R)

Anti-IL-23R antibody

DATASHEET

Host:Rabbit

Target Protein:IL-23R

IR:Immunogen Range:401-550/629

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:149233

Swiss Prot:Q5VWK5

Source:KLH conjugated synthetic peptide derived from human IL-23R:401-550/629 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Rat) Lysate at 40 ug
Lane 3: Thymus (Rat) Lysate at 40 ug
Primary: Anti-IL-23R (bs-1460R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65/75 kD
Observed band size: 75 kD

Sample: HL60 Cell Lysate at 30 ug
Primary: Anti- IL-23R (bs-1460R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 70 kD

Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-23R Polyclonal Antibody, Unconjugated(bs-1460R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining