Anti-Adenosine Receptor A2a antibody (bs-1456R)

Anti-Adenosine Receptor A2a antibody

DATASHEET

Host:Rabbit

Target Protein:Adenosine Receptor A2a

IR:Immunogen Range:165-300/412

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:135

Swiss Prot:P29274

Source:KLH conjugated synthetic peptide derived from human A2AR:165-300/412 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
MOlt4(Human) Cell Lysate at 30 ug
Raji Cell Lysate at 30 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD

Sample: placenta (Mouse) Lysate at 40 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45kD

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Thymus (Rat) Lysate at 40 ug
Lane 5: Lymph node (Rat) Lysate at 40 ug
Lane 6: SH-SY5Y (Human) cell Lysate at 30 ug
Lane 7: Molt-4(Human) cell Lysate at 30 ug
Lane 8: U87MG (Human) cell Lysate at 30 ug
Lane 9: Huvec (Human) cell Lysate at 30 ug
Primary: Anti-Adenosine Receptor A2a (bs-1456R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD