Anti-HA tag antibody (bs-0966R)

Anti-HA tag antibody

DATASHEET

Host:Rabbit

Target Protein:HA tag

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:N/A

Swiss Prot:N/A

Source:KLH conjugated HA Tag peptide: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:10000-30000)
ELISA(1:5000-10000)

Cross Reactive Species:HA tag Fusion Protein.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
HA-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary:
Anti-HA-Tag (bs-0966R) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

Sample:
Lane1: HA-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: HA-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-HA tag (bs-0966R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

Sample:
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug
Primary: Anti-HA tag (bs-0966R) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD