Anti-Vasohibin 1 antibody (bs-1505R)

Anti-Vasohibin 1 antibody

DATASHEET

Host:Rabbit

Target Protein:Vasohibin 1

IR:Immunogen Range:281-365/365

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:22846

Swiss Prot:Q7L8A9

Source:KLH conjugated synthetic peptide derived from human VASH1:281-365/365 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:VASH1 is a angiogenesis inhibitor. It inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. It does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis and acts in an autocrine manner. VASH1 also inhibits artery neointimal formation and macrophage infiltration and exhibits heparin-binding activity. There are two named isoforms.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- Vasohibin 1 (bs-1505R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Sample: Liver（Rat） lysate at 30ug;
Primary: Anti-alpha-Actinin-4 (bs-1505R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:3000 dilution;
Predicted band size : 40kD
Observed band size : 40kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min;
Incubation: Anti-VASH1 Polyclonal Antibody, Unconjugated(bs-1505R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining