Anti-CYP1A1 antibody (bs-1606R)

Anti-CYP1A1 antibody

DATASHEET

Host:Rabbit

Target Protein:CYP1A1

IR:Immunogen Range:361-460/512

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:57491

Swiss Prot:A9YTQ3

Source:KLH conjugated synthetic peptide derived from human CYP1A1:361-460/512 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooJNCgenase enzymes which require several cofactors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. EpoJNCgenases are those P450s which metabolize AA to epoJNCeicosatrienoic acid (EETs) and omega hydroJNClases are those P450s which produce 19- and 20-hydroJNCeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:K562 Cell (Human) Lysate at 30 ug
Primary: Anti- CYP1A1 (bs-1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD

Sample:Lung (Mouse) Lysate at 40 ug
Primary: Anti- CYP1A1 (bs-1606R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP1A1) Polyclonal Antibody, Unconjugated (bs-1606R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.