Anti-Profilin 1 antibody (bs-1556R)

Anti-Profilin 1 antibody

DATASHEET

Host:Rabbit

Target Protein:Profilin 1

IR:Immunogen Range:41-140/140

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5216

Swiss Prot:P07737

Source:KLH conjugated synthetic peptide derived from human Profilin 1:41-140/140 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Profilin 1 is a ubiquitous actin monomer-binding protein involved in actin polymerization in response to extracellular signals. Three profilin genes have been identified: profilin 1, 2, and 3. Profilin 1 is the most ubiquitous and abundant, and is highly expressed throughout development and adulthood inmost tissues including brain. Profilin 2 is the neuronal specific isoform and profilin 3 is a testis specific isoform. Profilins of eukaryotic cells are small (12-15 kDa) cytoplasmic proteins that bind to actin monomers, polyphosphoinositides and polymers of L-proline. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(3ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- Profilin 1 (bs-1556R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-PFN1 Polyclonal Antibody, Unconjugated(bs-1556R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Profilin 1 antibody (bs-1556R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.