Anti-Dopamine D4 receptor antibody (bs-1746R)

Anti-Dopamine D4 receptor antibody

DATASHEET

Host:Rabbit

Target Protein:Dopamine D4 receptor

IR:Immunogen Range:151-250/467

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1815

Swiss Prot:P21917

Source:KLH conjugated synthetic peptide derived from human DRD4:151-250/467 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats.DRD4 expression has been reported in various regions of the brain as well as in adrenal gland, artery, eye, heart, kidney, placenta, spinal cord, testis, and vas deferens.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
Urinary bladder (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D4 receptor (bs-1746R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41/51 kD
Observed band size: 51 kD

Sample:
A549(Human) Cell Lysate at 30 ug
MKN45(Human) Cell Lysate at 30 ug
Primary: Anti- Dopamine D4 receptor (bs-1746R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD

Sample:
Lane1: Brain(Mouse) Lysate at 30 ug
Lane2: Liver(Mouse) Lysate at 30 ug
Primary: Anti-DRD4(bs-1746R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 41kD
Observed band size : 41kD