Anti-PD-1 antibody (bs-1867R)

Anti-PD-1 antibody

DATASHEET

Host:Rabbit

Target Protein:PD-1

IR:Immunogen Range:201-288/288

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5133

Swiss Prot:Q15116

Source:KLH conjugated synthetic peptide derived from human PD-1:201-288/288 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
CD279 is an immunoglobulin superfamily member, also known as Programmed Cell Death 1. Programmed Cell Death 1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. Programmed Cell Death 1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The Programmed Cell Death 1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Raji(Human) Cell Lysate at 30 ug
Thymus(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 40 ug
Thymus (Rat) Lysate at 40 ug
Primary: Anti-PD-1 (bs-1867R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 58 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
MOLT-4(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-PD-1 (bs-1867R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 55 kD