Anti-IL2 Receptor beta antibody
| 英文名称 | IL2 Receptor beta |
| 中文名称 | 白介素2受体β链/IL-2Rβ 抗体 |
| 别 名 | Imterleukm-2 Receptor beta; CD 122; CD122; CD122 antigen; High affinity IL 2 receptor beta subunit; High affinity IL 2 receptor subunit beta; High affinity IL2 receptor beta subunit; High affinity IL2 receptor subunit beta; High affinity IL-2 receptor subunit beta; IL 2 receptor; IL-2 receptor; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IL15RB; IL2 R B; IL-2 R B; IL2 RB; IL2 receptor; IL2RB; Interleukin 2 receptor beta; Interleukin 2 receptor subunit beta; Interleukin-2 receptor subunit beta; OTTHUMP00000028799; P70 75; P7075; p75; IL2RB_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:IL2 Receptor beta
IR:Immunogen Range:501-551/551
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3560
Swiss Prot:P14784
Source:KLH conjugated synthetic peptide derived from human CD122:501-551/551
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-IL2 Receptor beta (bs-1959R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL2 Receptor beta) Polyclonal Antibody, Unconjugated (bs-1959R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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