Anti-CLEC5A antibody
| 英文名称 | CLEC5A |
| 中文名称 | C型凝集素结构域家族5成员A抗体 |
| 别 名 | A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; C-type lectin domain family 5 member A; C type lectin domain family 5 member A; C-type lectin superfamily member 5; CLEC5 A; CLEC5A; CLECSF 5; CLECSF5; MDL 1; MDL-1; MDL1; CLC5A_MOUSE; Myeloid DAP12-associating lectin 1; Myeloid DAP12 associating lectin. |
DATASHEET
Host:Rabbit
Target Protein:CLEC5A
IR:Immunogen Range:101-190/190
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:23845
Swiss Prot:Q9R007
Source:KLH conjugated synthetic peptide derived from mouse CLEC5A:101-190/190
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CLEC5A(bs-2663R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CLEC5A/MDL1 Polyclonal Antibody, Unconjugated(bs-2663R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell: U937.
Concentration: 1:100.
Incubation: 40 minutes, room temperature.
Host/Blank: U937 cells.
Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (bs-2663R) (green) compared with control in the absence of primary antibody (blue) followed by U937 cells.
secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)
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