Anti-CD171/L1CAM antibody (bs-1996R)

Anti-CD171/L1CAM antibody

DATASHEET

Host:Rabbit

Target Protein:CD171/L1CAM

IR:Immunogen Range:1101-1257/1257

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3897

Swiss Prot:P32004

Source:KLH conjugated synthetic peptide derived from human CD171:1101-1257/1257 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:L1cam (L1 cell adhesion molecule isoform 1 precursor) is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq].

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
Flow-Cyt(1μg /test)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD171 antibody(bs-1996R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions ).
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Antigen: bs-1996P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.

Antigen: bs-1996P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.