Anti-5-HTR3 antibody
| 英文名称 | 5-HTR3 |
| 中文名称 | 5-羟色胺受体3抗体 |
| 别 名 | 5 HT 3; 5 HT3; 5 HT3A; HTR-3A; 5 HT3A receptor; 5 HT3R; 5 HT3RA; 5 hydroJNCtryptamine (serotonin) receptor 3A; 5 HydroJNCtryptamine receptor 3; 5-HT-3; 5-HT3-A; 5-HT3A; 5-HT3R; 5-hydroJNCtryptamine receptor 3; 5-hydroJNCtryptamine receptor 3A; 5HT 3; 5HT 3A; 5HT receptor; 5HT3 serotonin receptor; 5HT3A_HUMAN; 5HT3R; HTR 3; HTR3; Htr3a; Serotonin gated ion channel receptor; Serotonin receptor 3; Serotonin receptor 3A; Serotonin-gated ion channel receptor 3a; 5HT3A_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:5-HTR3
IR:Immunogen Range:301-400/484
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3359
Swiss Prot:P46098
Source:KLH conjugated synthetic peptide derived from human 5-HTR3:301-400/484
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroJNCtryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:100-200)
IHC-F(1:100-200)
IF(1:50-100)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Spinal cord (Mouse) Lysate at 40 ug
Lane 4: Heart (Mouse) Lysate at 40 ug
Lane 5: Heart (Rat) Lysate at 40 ug
Primary: Anti-5-HTR3 (bs-2126R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52-55 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5-HTR3) Polyclonal Antibody, Unconjugated (bs-2126R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5-HTR3) Polyclonal Antibody, Unconjugated (bs-2126R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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