Anti-CD69 antibody (bs-2499R)

Anti-CD69 antibody

DATASHEET

Host:Rabbit

Target Protein:CD69

IR:Immunogen Range:101-199/199

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:969

Swiss Prot:Q07108

Source:KLH conjugated synthetic peptide derived from human CD69:101-199/199 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (bs-2499R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 34 kD

Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD