> 免疫组学 > 抗原和抗体 > 多克隆抗体 > Anti-phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184) antibody (bs-3027R)Anti-phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184) antibody
| 英文名称 | phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184) |
| 中文名称 | 磷酸化腺苷单磷酸活化蛋白激酶β1抗体 |
| 别 名 | phospho-AMPK beta 1 + AMPK beta 2 (Ser182+Ser184); PRKAB1(phospho S182); PRKAB1(phospho-S182); AMPK beta 1(phospho Ser182); p-AMPK beta 1(Ser182); p-AMPK beta 1(S182); AMPK beta 2(phospho Ser184); 5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta -1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1. |
DATASHEET
Host:Rabbit
Target Protein:phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184)
IR:Immunogen Range:SS(p-S)PP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5564
Swiss Prot:Q9Y478
Source:KLH conjugated synthesised phosphopeptide derived from human AMPK beta 1 around the phosphorylation site of Ser182:SS(p-S)PP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboJNClase (ACC) and beta-hydroJNC beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(2ug/Test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-AMPK beta 1 + AMPK beta 2 (Ser182/Ser184)) Polyclonal Antibody, Unconjugated (bs-3027R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-AMPK beta 1(Ser182) Polyclonal Antibody, Unconjugated(bs-3027R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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