Anti-Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) antibody (bs-3073R)

Anti-Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)

IR:Immunogen Range:TA(p-S)EP

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9759

Swiss Prot:P56524

Source:KLH conjugated Synthesised phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246:TA(p-S)EP 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)(bs-3073R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kD
Observed band size: 119 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.