Anti-Phospho-FoxO3a (Ser318 + Ser321) antibody
| 英文名称 | Phospho-FoxO3a (Ser318 + Ser321) |
| 中文名称 | 磷酸化叉头蛋白3A抗体 |
| 别 名 | AF6q21; AF6q21 protein; DKFZp781A0677; FKHR2; FKHRL 1; FKHRL1; FKHRL1P2; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1; Forkhead box O3; Forkhead box O3A; Forkhead box protein O3; Forkhead box protein O3A; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1; Forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma like 1; Forkhead in rhabdomyosarcoma-like 1; FOX O3A; FOXO2; foxo3; FOXO3_HUMAN; FOXO3A; MGC12739; MGC31925. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-FoxO3a (Ser318 + Ser321)
IR:Immunogen Range:NA(p-S)TV(p-S)GR
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2309
Swiss Prot:O43524
Source:KLH conjugated Synthesised phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser318/321:NA(p-S)TV(p-S)GR
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-FoxO3a (Ser318 + Ser321) (bs-3138R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-FoxO3a (Ser318 + Ser321)) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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