Anti-phospho-GSK-3 Beta (Thr390) antibody
| 英文名称 | phospho-GSK-3 Beta (Thr390) |
| 中文名称 | 磷酸化糖原合酶激酶3β抗体 |
| 别 名 | GSK3B(Phospho-Thr390); GSK3B(Phospho-T390); p-GSK-3 Beta(Thr390); p-GSK-3 beta(T390); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-GSK-3 Beta (Thr390)
IR:Immunogen Range:AS(p-T)PT
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2932
Swiss Prot:P49841
Source:KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Thr390:AS(p-T)PT
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg/Test)
IF(1:100-500)
Cross Reactive Species:Human
Rat
Chicken
Dog
Pig
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Liver (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Pancreas (Rat) Lysate at 40 ug
Primary: Anti-phospho-GSK-3 Beta (Thr390) (bs-3148R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GSK-3 Beta(Thr390)) Polyclonal Antibody, Unconjugated (bs-3148R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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