Anti-CD44v5 antibody (bs-2782R)

Anti-CD44v5 antibody

DATASHEET

Host:Rabbit

Target Protein:CD44v5

IR:Immunogen Range:61-139/139

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:960

Swiss Prot:P16070

Source:KLH conjugated synthetic peptide derived from human CD44v5:61-139/139 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1μg /Test)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD44v5 Polyclonal Antibody, Unconjugated(bs-2782R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining