Anti-Phospho-FGFR1 (Tyr653 + Tyr654) antibody
| 英文名称 | Phospho-FGFR1 (Tyr653 + Tyr654) |
| 中文名称 | 磷酸化碱性成纤维细胞生长因子受体1抗体 |
| 别 名 | FGFR1 alpha (phospho Y654) ; FGFR1 (phospho Y653 + Y654); p-FGFR1 (phospho Y653 + Y654); bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; FGF Receptor; CEK; FGFBR; FGFR 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fms like tyrosine kinase 2; Fms related tyrosine kinase 2; Fms related tyrosine kinase 2 Pfeiffer syndrome; H2; H3; H4; H5; HBGFR; Heparin binding growth factor receptor; HydroJNCaryl protein kinase; KAL 2; KAL2; MFR; N SAM; N sam tyrosine kinase; Protein tyrosine kinase; Tyrosylprotein kinase; Basic fibroblast growth factor receptor 1. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-FGFR1 (Tyr653 + Tyr654)
IR:Immunogen Range:ID(p-Y)(p-Y)KK
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2260
Swiss Prot:P11362
Source:KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr653/654:ID(p-Y)(p-Y)KK
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-FGFR1(Tyr653 + Tyr654) (bs-3205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 128 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- Phospho-FGFR1(Tyr653 + Tyr654) (bs-3205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 128 kD
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-FGFR1 (Tyr653 + Tyr654) Polyclonal Antibody, Unconjugated(bs-3205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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