Anti-phospho-LKB1 (Ser428) antibody
| 英文名称 | phospho-LKB1 (Ser428) |
| 中文名称 | 磷酸化丝氨酸/苏氨酸蛋白激酶抗体 |
| 别 名 | STK11(phospho S428); LKB1 (phospho S428); p-LKB1 (phospho S428); LKB1(phospho Ser428); p-LKB1(phospho Ser428); p-LKB1(S428); LKB1; PJS; Renal carcinoma antigen NY-REN-19; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine protein kinase LKB1; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Stk11; STK11_HUMAN; hLKB1; Liver kinase B1; LKB 1; NY REN 19 antigen; Peutz Jeghers syndrome; PJS; Polarization related protein LKB1; Serine/threonine protein kinase STK11; Serine/threonine-protein kinase XEEK1; STK 11. |
DATASHEET
Host:Rabbit
Target Protein:phospho-LKB1 (Ser428)
IR:Immunogen Range:RL(p-S)AC
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6794
Swiss Prot:Q15831
Source:KLH conjugated Synthesised phosphopeptide derived from human LKB1 around the phosphorylation site of Ser428:RL(p-S)AC
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-LKB1(Ser428) (bs-3249R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LKB1(Ser428)) Polyclonal Antibody, Unconjugated (bs-3249R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LKB1(Ser428)) Polyclonal Antibody, Unconjugated (bs-3249R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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