Anti-Phospho-MSK1 (Ser360) antibody
| 英文名称 | Phospho-MSK1 (Ser360) |
| 中文名称 | 磷酸化有丝分裂原和应激活化型蛋白激酶1抗体 |
| 别 名 | MSK1 (phospho S360); MSK1 (Phospho-Ser360); MSK1 (Phospho-S360); p-MSK1 (Ser360); Pp-MSK1 (S360); MSK1; RPS6KA5; 90 kDa ribosomal protein S6 kinase 5; EC 2.7.11.1; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-MSK1 (Ser360)
IR:Immunogen Range:TY(p-S)PA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9252
Swiss Prot:O75582
Source:KLH conjugated synthesised phosphopeptide derived from human MSK1 around the phosphorylation site of Ser360:TY(p-S)PA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Cow
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Lymph node lyates(mo);
Primary: Rabbit Anti-Phospho-MSK1 (Ser360) (bs-3281R) at 1:300 dilution;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:90 kD Observed band size:100 kD
Tissue/cell: Mouse bain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MSK1 Polyclonal Antibody, Unconjugated(bs-3281R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MSK1 (Ser360)) Polyclonal Antibody, Unconjugated (bs-3281R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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