Anti-Phospho-NDRG1 (Thr346) antibody
| 英文名称 | Phospho-NDRG1 (Thr346) |
| 中文名称 | 磷酸化分化相关基因NDRG1抗体 |
| 别 名 | NDRG1 (Phospho Thr346); NDRG1 (Phospho T346); p-NDRG1 (Thr346); N-myc downstream regulated gene 1; TDD5; 42 kDa; cap43; cmt4d; Differentiation related gene1 protein; Drg 1; drg1; gc4; hmsnl; Human mRNA for RTP complete cds; N myc downstream regulated gene 1 protein; Ndr 1; NDRG 1; Nickel specific induction protein Cap43; Nmyc downstream regulated gene1; Protein NDRG1; Protein regulated by oJNCgen 1 ; Protein regulated by oJNCgen1; proJNC1; reducin; Reducing agents and tunicamycin responsive protein; rit42; rtp; targ1; tdds; tunicamycin-responsive protein. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-NDRG1 (Thr346)
IR:Immunogen Range:SH(p-T)SE
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:10397
Swiss Prot:Q92597
Source:KLH conjugated Synthesised phosphopeptide derived from human NDRG1 around the phosphorylation site of Thr346:SH(p-T)SE
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (NDRG1 (Thr346)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3298R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (NDRG1 (Thr346)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3298R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (NDRG1 (Thr346)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3298R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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